Fig. 6.

Knockdown of NEAT1 sensitized GC cells to radiotherapy by sponging miR-27b-3p in vivo. MKN-45 cells stably transfected with shNEAT1, scrambled, shNEAT1 + anti-miR-NC, or shNEAT1 + anti-miR-27b-3p were subcutaneously inoculated into nude mice, which received 4 Gy radiation treatment a week. 21 days upon radiation treatment, the tumors were resected and weighted. Tumor volumes (a) and mice body weight (b) were measured every 3 days after xenograft tumor was irradiated with 4 Gy X-rays. c Tumors were weighted at 21 days of radiation. d qRT-PCR was conducted to examine the expression of NEAT1 and miR-27b-3p in tumor tissues of MKN-45 cells implanted into nude mice. *P < 0.05