Table 2.

Primers and probes used for plasmid construction and real-time quantitative PCR

	Sequence (5′ → 3′)	Annotation
fib-F	GAATTCGCAGGGAAATCAAACGGG	PCR for pPIC9K-fib
fib-R	GCGGCCGCTTAGTGGTGATGATGGTGATGTTGAGCAGCAGCTTGAACGT	PCR for pPIC9K-fib
fib-F	GCAGGGAAATCAAACGGG	PCR for pMD19T-fib
fib-R	TTGAGCAGCAGCTTGAACGT	PCR for pMD19T-fib
TDH1-F	ATGGCTATCACTGTCGGTATTAACG	PCR for pMD19T-TDH1
TDH1-R	TTAAGCCTTAGCAACGTGTTG	PCR for pMD19T-TDH1
qfib-F	TTGCCGGTGGAGCTTCTATG	qPCR for fib
qfib-R	CAACAGAGTTGTTCAAAGCAGCA	qPCR for fib
Probe-fib	HEX-TCCATTCCAAGATTACAACTCTCATGG-BHQ-1	qPCR for fib
qTDH1-F	CGGTGTTTTCACCACTTTGGA	qPCR for TDH1
qTDH1-R	CAACGAACATTGGAGCATCCT	qPCR for TDH1
Probe-TDH1	HEX-CCAAAAGCACATCGACGCCGGT-BHQ-1	qPCR for TDH1