Table 3.
Strain | Fibase activity | Ct value | Gene copy (10n) | Copy numbers of fib gene (fib/TDH1 copy number) | ||
---|---|---|---|---|---|---|
fib gene | TDH1 gene | fib gene | TDH1 gene | |||
GS115/ fib-5.4c | 4428 | 26.00 ± 0.12 | 26.22 ± 0.59 | 18.6 ± 1.46 | 3.45 ± 1.20 | 5.4 |
GS115/ fib-6c | 5781 | 16.13 ± 0.02 | 16.99 ± 0.23 | 14.9 ± 1.88 | 2.50 ± 0.42 | 6.0 |
GS115/ fib-8c | 7323 | 16.00 ± 0.19 | 16.40 ± 0.24 | 13.4 ± 0.14 | 1.68 ± 0.27 | 8.0 |
GS115/ fib-9c | 7930 | 16.23 ± 0.04 | 17.19 ± 0.07 | 12.6 ± 0.32 | 1.45 ± 0.07 | 8.7 |
GS115/ fib-12c | 2472 | 26.35 ± 0.19 | 27.71 ± 0.19 | 14.8 ± 1.88 | 1.21 ± 0.15 | 12.3 |
Data are presented as the mean ± SD of triplicate determinations