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. 2020 Nov 23;21(1):69. doi: 10.3892/etm.2020.9501

Figure 3.

Figure 3

Structure of the ASwalR transcript and association with walR mRNA. (A) Detection of the 5'-terminus of the ASwalR transcript by 5'-RACE. The PCR product size was ~120 bp. (B) Transcript amounts of ASwalR in Enterococcus faecalis cultures were observed in a representative northern blot. The loading sample without RNA was used as the blank control. (C) Schematic of the ASwalR 5'-terminus showing that transcription starts within the walR ORF and is complementary to most of the walR ORF. The sequence of the first strand synthesis ASwalR RT-PCR amplicon was from 1159871-1160384 bp. The overlapping sequence from first strand-specific ASwalR RT-PCR and 5'-RACE product (89 bp) was from 1159871-1159960 bp in the genome. The full length for ASwalR RNA is 550 bp (1159834-1160384 bp). ASwalR, walR antisense RNA; ORF, open reading frame; RT-PCR, reverse transcription-PCR; 5'-RACE, 5'-rapid amplification of cDNA ends; AS, antisense.