Figure 2.

BBM inhibits the proliferation of triple negative breast cancer cells. Cells were treated with applied concentrations (10, 20 and 40 µM) of BBM. (A) The cell proliferation was tested using a colony formation assay and medium was renewed every 3 days. (B) Quantification of colony forming assay results. (C) EdU assay images and (D) quantification (scale bar, 100 µm). The Ctrl group was untreated cells. Data are presented as the mean ± standard deviation (n=3). *P<0.05, **P<0.01, ***P<0.001 vs. Ctrl group. BBM, Berbamine; Ctrl, control.