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. 2020 Nov 25;21(1):71. doi: 10.3892/ol.2020.12332

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Copyright: © Iida et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — SFN inhibits expression levels of SLC7A11. H69 cells were treated with SFN (20 µM) for 96 h or untreated, as a control. (A) Decreases in mRNA expression levels of SLC7A11 following SFN treatment were determined using reverse transcription-quantitative PCR. Expression levels were normalized to those of the untreated control group. Data are presented as the mean ± SD (n=6). ****P<0.0001. (B) The expression levels of SLC7A11 protein were detected using western blot analysis. The band obtained at 96 h was quantified with ImageJ software and intensity was normalized to that of β-actin. Data are presented as the mean ± SD (n=5). ***P<0.001. (C) Change in glutathione concentration following SFN treatment was determined using a glutathione assay. Data are presented as the mean ± SD (n=9). ***P<0.001. SFN, sulforaphane; SLC7A11, cystine/glutamate antiporter xCT.