Table 1.
Phenotypic and genotypic features of strain ElPT compared to closely related strains. The genome analysis is based on GenBank accession numbers for strain ElPT (CP036426–CP036431), Tautonia sociabilis (GCA_003977685.1), Isosphaera pallida (CP002353–CP002354), Singulisphaera acidiphila (CP003364–CP003367), Paludisphaera borealis (CP019082–CP019084) and Aquisphaera giovannonii (CP042997–CP042999). The genome of Tundrisphaera lichenicola has not been sequenced yet
| Feature | ElPT |
Tautonia sociabilis GM2012T |
Isosphaera pallida IS1BT |
Tundrisphaera lichenicola P12T |
Singulisphaera acidiphila MOB10T |
Paludisphaera borealis PX4T |
Aquisphaera giovannonii OJF2T |
|---|---|---|---|---|---|---|---|
| Phenotypic characteristics | |||||||
| Shape | Spherical | Spherical | Spherical | Spherical | Spherical | Spherical | Spherical |
| Diameter (µm) | 1.7 ± 0.3 | 1.7–2.9 | 2.5–3.0 | 2.2–3.0 | 1.6–2.6 | 1.5–2.5 | 1.6–2.0 |
| Colour | Pink | White | Pink | Pink | White | Bright pink | Pink |
| Relation to oxygen | Aerobic | Strictly aerobic | Strictly aerobic | Strictly aerobic | Strictly aerobic | Aerobic | Strictly aerobic |
| Temperature range (optimum) (°C) | 10–33 (30) | 37–46 (42) | 34–55 (41) | 4–28 (15–22) | 4–33 (20–26) | 6–30 (15–25) | 10–35 (30) |
| pH range (optimum) | 6–0-8.5 (7.5) | 5.5–9.0 (7.5) | 7.8–8.8 | 4.5–6.8 (5.5–6.0) | 4.2–7.5 (5.0–6.2) | 3.5–6.5 (5.0.-5.5) | 6.5–9.5 (7.5–8.5) |
| Division | Budding | Budding | Budding | Budding | Budding | Budding | Budding |
| Dimorphic life cycle | n.o. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| Motility | No | No | Yes, phototactic gliding | No | No | No | No |
| Crateriform structures | Ubiquitous | n.d. | Ubiquitous | Ubiquitous | Ubiquitous | Ubiquitous | Ubiquitous |
| Fimbriae | Yes | n.d. | Yes | n.d. | n.d. | n.d. | Yes |
| Capsule | Yes | n.d. | No | n.d. | Yes | n.d. | Yes |
| Stalk | n.o. | n.d. | n.d. | No | No | n.d. | n.d. |
| Holdfast structure | n.o. | n.d. | No | Yes | Yes | Yes | n.d. |
| Genomic characteristics | |||||||
| Genome size (bp) | 9,395,224 | 6,760,005 | 5,529,304 | n.d. | 9,755,686 | 7,651,896 | 10,526,296 |
| Plasmids | 5 | n.d. | 1 | n.d. | 3 | 2 | 2 |
| G + C (%) | 71.1 ± 0.8 | 70.1 | 62.5 ± 3.2 | 61.2–62.2 | 62.2 ± 2.3 | 66.3 ± 4.1 | 70.8 ± 0.5 |
| Coding density (%) | 84.7 | 85.0 | 84.7 | n.d. | 83.5 | 86.1 | 85.7 |
| Completeness (%) | 98.28 | 98.28 | 98.28 | n.d. | 98.28 | 96.55 | 96.55 |
| Contamination (%) | 5.17 | 3.45 | 0 | n.d. | 6.90 | 3.45 | 5.17 |
| Total genes | 7707 | 5183 | 3828 | n.d. | 7689 | 5961 | 7953 |
| Genes/Mb | 820 | 767 | 692 | n.d. | 788 | 779 | 756 |
| Giant genes | 0 | 0 | 0 | n.d. | 1 | 0 | 1 |
| All protein-coding genes | 7556 | 5084 | 3761 | n.d. | 7540 | 5855 | 7835 |
| Protein-coding genes/Mb | 804 | 752 | 680 | n.d. | 773 | 765 | 744 |
| Hypothetical proteins | 3399 | 3175 | 1821 | n.d. | 4316 | 3154 | 3328 |
| tRNAs | 100 | 84 | 51 | n.d. | 81 | 83 | 107 |
| 16S rRNA genes | 3 | 1 | 3 | n.d. | 8 | 3 | 3 |
n.o. not observed, n.d. not determined