Fig. 1.

MiR-4490 is downregulated in GC cells and tissues. (a) Expression of miR-4490 in human GC-derived cells compared with human normal gastric epithelial GES-1 cells. **, p < 0.05. (b) Relative expression of miR-4490 measured using qRT-PCR in 70 pairs of GC samples and corresponding non-cancerous samples; the results were normalized to endogenous U6 RNA. **, p < 0.05. (c) Expression of miR-4490 in paired GC tissues and matched normal tissues. (d) Expression of miR-4490 in different clinical GC stages. The patients were staged in accordance with the 7th Edition of the AJCC Cancer Tumor Node Metastasis Classification. **, p < 0.05. stages I-II vs. stages III-IV. (e) The miR-4490 expression level is lower in T3–4 than in T1–2 GC samples. **, p < 0.05. (f) ISH analysis of miR-4490 expression in normal gastric mucosa and GC tissues. Data are shown as the means ± SD from at least three independent experiments. Scale bars, 50 μm in F