Fig. 3.

MiR-4490 suppresses GC cell migration/invasion via EMT in vitro. (a) Scratch wound healing assays were used to detect GC cell motility following transfection with NC and miR-4490 mimics or a miR-4490 inhibitor. Wound closure percentages are shown in the top panel. ****, p < 0.001. (b) Representation and quantification of matrigel assay indicating the invasive capacity of GC cells transfected with NC, miR-4490 mimics, or a miR-4490 inhibitor. ****, p < 0.001. Error bars represent the means ± SD from three independent experiments. (c) GC cell morphology observed under an inverted phase-contrast microscope. (d) Expression levels of E-cadherin, γ-cadherin, vimentin, ZEB1, N-cadherin and MMP2 in GC cells analyzed by Western blotting. GAPDH was used as a loading control. (e) Immunofluorescence analysis of E-cadherin and vimentin expression in human GC cells. Scale bars, 200 μm in C and 20 μm in E