Fig. 5.

MiR-4490 suppresses USP22-mediated GC cell growth and invasion in vivo. (a) Representative images of tumors in nude mice after inoculation of BGC-823 cells stably expressing LV-m-NC, LV-miR-4490, LV-vector, LV-USP22 and LV-4490/USP22 (n = 3). (b) Tumor volumes measured after GC cell inoculation in each group. ****, p < 0.001, m-NC vs. miR-4490; ****, p < 0.001, miR-4490 vs. miR-4490/USP22. (c) and (d) Immunohistochemical (IHC) detection and quantification of Ki-67 expression in subcutaneous tumors from mice injected with GC cells. Student’s t test. ****, p < 0.001, m-NC vs. miR-4490; ***, p < 0.01, miR-4490 vs. miR-4490/USP22 and Ki-67. (e) Lungs of mice orthotopically transplanted with GC cells (n = 3 in each group). (f) Quantification of the number of metastatic loci in the lungs. ***, p < 0.01, m-NC vs. miR-4490; **, p < 0.05, miR-4490 vs. miR-4490/USP22. (g) E-cadherin expression in tumors derived from GC cells measured by qRT-PCR; **, p < 0.05, m-NC vs. miR-4490 and miR-4490 vs. miR-4490/USP22. (h) Immunohistochemical (IHC) detection of E-cadherin and vimentin expression in lung metastases. Scale bars, 200 μm in C and H