Table 3.
Affinity of Chelator–Antibody Conjugates and Efficiency of Conjugation
| Antibodya | Affinity (KD, nM) |
Affinity Reduction Upon Conjugation (Conj Ab KD/Unconj Ab KD)b |
MALDIc MW (Da) |
Efficiency of Conjugation (DTPAd:IgG)e |
|---|---|---|---|---|
| αF1Ig 2 | 0.23±0.02 | — | 147,632±39 | — |
| αF1Ig 2–DTPA | 0.21±0.03 | 0.9 | 148,648±393 | 1.9±0.7 |
| αF1Ig 8 | 0.08±0.00 | — | 145,213±58 | — |
| αF1Ig 8–DTPA | 0.19±0.03 | 2.4 | 145,841±98 | 1.2±0.3 |
Notes: aαF1Ig = anti-F1 IgG1. The number indicates the specific clone. b≤1 = no affinity reduction. cMALDI, matrix-assisted laser-desorption ionization. dDTPA, S-2-(4-isothiocyanatobenzyl)-diethylenetriamine pentaacetic acid. e(MWconjugated IgG–MW IgG)/MW conjugated DTPA (541 Da).