KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-FLAG | Sigma-Aldrich | Cat#F7425; RRID:AB_439687 |
| Mouse monoclonal anti-OctA-Probe (F-tag-01) | Santa Cruz | Cat#sc-51590; RRID:AB_677316 |
| Mouse monoclonal anti-Pgk1 (22C5D8) | Thermo Fisher | Cat#459250; RRID:AB_2532235 |
| Donkey polyclonal anti-Mouse Secondary | Thermo Fisher | Cat#A24506; RRID:AB_2535975 |
| Donkey anti-Rabbit Secondary | Sigma-Aldrich | Cat#GENA934; RRID:AB_2722659 |
| Mouse monoclonal anti-HA | Sigma-Aldrich | Cat#H3663; RRID:AB_262051 |
| Phospho-S6 Ribosomal Protein (Ser235/236) | Cell Signaling | Cat#4858; RRID:AB_916156 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Protease inhibitor tablets, EDTA-free | Thermo Fisher | Cat#A32965 |
| dCTP, [α−32P]-6000Ci/mmol | Perkin Elmer | Cat#BLU513Z |
| MG-132 | Sigma-Aldrich | Cat#474790 |
| RNasecure | Thermo Fisher | Cat#7006 |
| Synthetic HA-Luc19: MGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAMEDAKNIKKGPAPFYPLED | GenScript | This paper |
| Critical Commercial Assays | ||
| Random Primed DNA Labeling Kit | Sigma-Aldrich | Cat#11004760001 |
| Experimental Models: Organisms/Strains | ||
| S. cerevisiae: upf1Δ (HFY871): MATa ade2–1 his3–11,15 leu2–3,112 trp1–1 ura3–1 can1–100 upf1::HIS3 | Jacobson lab | He et al., 1997 |
| S. cerevisiae: pab1Δ pbp1Δ upf1Δ: MATa ade2–1 his3–11,15 leu2–3,112 trp1–1 ura3–1 can1–100 pab1::HIS3 pbp1::LEU2 upf1::KanMX6 | Jacobson lab | This paper |
| S. cerevisiae: pab1ΔC upf1Δ: MATa ade2–1 his3–11,15 Ieu2–3,112 trp1–1 ura3–1 can1–100 pab1ΔAC::HIS3 upf1::KanMX6 | Jacobson lab | This paper |
| S. cerevisiae: pbp1Δ upf1Δ: MATa ade2–1 his3–11,15 leu2–3,112trp1–1 ura3–1 can1–100 pbp1::LEU2 upf1::KanMX6 | Jacobson lab | This paper |
| Software and Algorithms | ||
| MultiGauge software | Fujifilm | Science lab 2005 |