Fig. 1.

Effect of sirolimus (SIR) versus torin2 on mechanistic target of rapamycin complex (mTORC)1 [phosphorylated eukaryotic translation initiation factor 4E-binding protein 1 (p4E-BP1)] and mTORC2 [phosphorylated Akt (pAkt) (Ser⁴⁷³)] in PKD1^−/− cells. A: PKD1^+/+ [renal cortical tubular epithelium (RCTE)] and PKD1^−/− (WT 9-12) cells were treated with sirolimus (10 nM) or torin2 (100 nM), and the effect on substrates of mTORC1 signaling [p4E-BP1 (Ser⁶⁵, Thr^37/46, and Thr⁷⁰)] and mTORC2 [pAkt (Ser⁴⁷³)] was determined by immunoblot analysis. Values of the ratio of total to phosphorylated protein abundance are means ± SE; n = 3 per group in triplicate. B: MTT assay of PKD1^+/+ and PKD1^−/− cells treated with sirolimus or torin2. OD, optical density (590 nm). Values are means ± SE; n = 4 per group, in triplicate. *P < 0.05, **P < 0.01, and #P < 0.0001 (by ANOVA with a Newman-Keuls post test).