Fig. 3.
Effect of sirolimus (SIR) versus torin2 on apoptosis (TUNEL) and proliferation [proliferating cell nuclear antigen (PCNA)]. A: PCNA and TUNEL staining in noncystic tubules from wild-type (WT) and Pkd1RC/RC [polycystic kidney disease (PKD)] kidneys, expressed as the percentage of cells staining positive per 240-µm2 area (%staining cells). B: PCNA and TUNEL staining in cells lining the cysts in PKD kidneys, expressed as the percentage of cells lining the cyst staining positive per cyst (%cyst-lining cells). White arrows indicate 3,3′-diaminobenzidine tetrahydrochloride hydrate-positive cells. VEH, vehicle. Scale bars = 50 µm. Values are means ± SE. *P < 0.05, **P < 0.01, and ***P < 0.0001 (by ANOVA with a Newman-Keuls post test).