Fig. 2.

The effect of acute high-intensity exercise and short-term high-intensity interval training (HIIT) on skeletal muscle and plasma humanin levels. Vastus lateralis humanin absolute (A) and relative (B) muscle levels, and absolute (C) and relative (D) plasma levels at rest (Pre), after five exercise intervals (Mid), immediately (Post) and 4 h (4 h) following acute high-intensity exercise before (untrained) and after (trained) short-term HIIT. Horizontal dotted line represents pre-exercise. Representative Western blots (A and B) are from two different participants. Significant main 2-way ANOVA effects are given in figures and *P < 0.05 for post hoc vs. Pre of same training status.