The effect of acute high-intensity exercise and short-term term high-intensity interval training (HIIT) on skeletal muscle and plasma SHLP levels. Absolute and relative plasma SHLP2 (A, B) and SHLP6 (C, D) concentrations at rest (Pre), after five exercise intervals (Mid), immediately (Post) and 4 h (4 h) following acute high-intensity exercise before (untrained) and after (trained) HIIT. Horizontal dotted line represents Pre level. Significant main 2-way ANOVA effects are given in figures and #P < 0.05 for post hoc vs. untrained at same time-point. SHLP, small humanin-like peptides.