Fig. 5.

mRNA levels of MT-RNR2 and humanin following acute high-intensity exercise untrained. Primers were used against sequences within the MT-RNR2 gene that were independent of (MT-RNR2 primer) or matched to the humanin mRNA sequence (humanin primer) (A). MT-RNR2 mRNA muscle levels (B) and association with humanin muscle levels (C) in response to untrained acute high-interval exercise. Muscle mRNA from acute high-intensity exercise pretraining was fractioned into small (<200 nucleotides) and large (>200 nucleotides) mRNA fragments (D), and these fragments were assayed with the humanin primer probe (E, F). Curved dotted lines represent 95% confidence intervals (C). Horizontal dotted line represents Pre level (E, F). n = 7–8 for small mRNA faction assay due to limited amount of sample available. *P < 0.05 ***P < 0.01 vs. for one-way ANOVA.