Fig. 5.

Adult male offspring administered low protein (LP) during the last week of gestation (LP12.5) exhibit glucose intolerance and insulin resistance in high-fat (HF) diet. Intraperitoneal glucose tolerance tests at 6 (A), 10 (B), and 14 (C) wk in HF [A and B: area under the curve (AUC), P = 0.05; C: AUC, P = 0.0063] and insulin tolerance tests at 16 wk in HF (D, AUC, P = 0.0238) of male LP12.5 and control (Ctrl) mice. In vivo glucose stimulated-insulin secretion (GSIS, E) and β-cell mass (F). Representative islets in male LP12.5 and Ctrl mice at 18 wk in HF diet (G, scale 100 μM). Analyzed by Student’s t test, 2-tailed and unpaired, *P < 0.05 vs. Ctrl, n = 4–6.