Fig. 1.
In PC-KO mice, GABAA-mediated inhibitory postsynaptic currents (IPSCs) are selectively lost in Purkinje cells (PCs). Spontaneous IPSCs recorded from Purkinje cells (subsequently blocked by gabazine, gray traces) in PC-Bl6 (A), PC-Mixed (B), and Global (C) wild-type (WT), hemizygous (HEMI), and knockout (KO) animals. D–F: IPSCs recorded from molecular layer interneurons. Insets: average molecular layer interneuron IPSCs (normalized amplitude) before (black) or in the presence of zolpidem (teal traces) for KO animals. Light shading indicates SE. Note that IPSCs are lost in Purkinje cells in KO animals, but are unaffected in PC-KO molecular layer interneurons. Gray bars in ii columns indicate means. Scale bars: A–C: 100 pA; 200 ms. D–F: 50 pA; 200 ms; 5 ms, insets. See also Table 1. Freq., frequency.