Fig 4.
Association of SP cells with axons derived from excitatory interneurons that express different neuropeptides. (A–C) Show fields from Tac1Cre mice that had received AAV.BB2 injections and had then been reacted with antibodies against TFP, Homer, VGLUT2 and either SP (A), neurotensin (NTS, (B)) or NKB (C). The layout is equivalent to that shown in parts A-D of Fig. 2. (A) A TFP-labelled dendrite contains a Homer punctum (arrowhead), which is associated with a VGLUT2 bouton that lacks SP. A nearby SP-positive VGLUT2 bouton is indicated with an arrow. (B) A small Homer punctum on a TFP-labelled dendritic spine (arrow) is adjacent to a NTS-positive VGLUT2 bouton. (C) A TFP dendrite and attached dendritic spine have two Homer puncta. One of these (arrow) is adjacent to a VGLUT2 bouton with strong NKB-immunoreactivity, while the other (arrowhead) is in contact with a VGLUT2 bouton that shows very weak NKB labelling. Scale bar (A–C) = 2 μm.