Table 1.
Serology and Cross-Reactivity Using a SARS-CoV-2 Commercial ELISA Test in Patients With Tropical Endemic Diseases From Córdoba, Colombia, and According Assay Manufacturers
| Patient’s Group | N | Diagnosis Period | Positive Samples by ELISA COVID-19 Test (%) | Negative Samples by ELISA COVID-19 Test (%) | |
|---|---|---|---|---|---|
| Patients from Córdoba, Colombia | Confirmed SARS-CoV-2 infectiona | 63 | June–July 2020 | 58 (92) | 5 (8) |
| COVID-19 clinically diagnosed and asymptomatic contactsb | 8 | July 2020 | 6 (75) | 2 (25) | |
| Acute dengue infectionc | 8 | April–December 2019 | 0 (0) | 8 (100) | |
| Recent dengue infectionc | 15 | April–December 2019 | 0 (0) | 15 (100) | |
| Acute Zika infectiond | 19 | November 2015–February 2016 | 5 (26) | 14 (74) | |
| Previous Chikungunya infectione | 9 | November 2015–April 2016 | 0 (0) | 9 (100) | |
| Recent exposure to spotted fever group Rickettsiaef | 5 | February 2013 | 0 (0) | 5 (100) | |
| Data according assay manufacturers (Ingenasa; Eurofins, Madrid, Spain)g | Confirmed RT-qPCR SARS-CoV-2 infection | 162 | Ongoing SARS-CoV-2 pandemic | 150 (92.6) | 12 (7.4) |
| Healthy patients/non-SARS-CoV-2 infection | 249 | Before SARS-CoV-2 pandemic | 2 (0.8) | 247 (99.2) | |
| Seasonal coronavirus (229E, NL63, OC43 HKU1) infection | 121 | No data | 0 (0) | 121 (100) |
Abbreviations: COVID-19, coronavirus disease 2019; ELISA, enzyme-linked immunosorbent assay; RT-qPCR, quantitative reverse-transcription polymerase chain reaction; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.
aSerum samples were collected 2 weeks before (in 32 patients) and 2 weeks after (in 31 patients) the date of SARS-CoV-2 RT-qPCR-positive collected swab sample.
bSerum samples of symptomatic patients were collected during the first 2 weeks of symptoms in 1 patient and 2 weeks after symptoms in 5 patients.
cSerum samples were collected on the same day as dengue-test (RT-qPCR, NS1 antigen, ELISA immunoglobulin [Ig]M) positive samples.
dSerum samples were collected on the same day as Zika-RT-qPCR-positive samples.
eSerum samples were collected the on same day as Chikungunya-ELISA IgG-positive samples.
fSerum samples were collected the same day as spotted fever group Rickettsiae-immunofluorescence assay-IgG positive samples.