Figure 2.

Mode of Leishmania infantum TryS (LiTryS) inhibition by MOL2008. Lineweaver–Burk reciprocal plots for the LiTryS activity measured at different inhibitor (black square: 0 µM, red circle: 0.15 µM, blue triangle: 0.375 µM, violet star: 0.75 µM, green inverted triangle: 1.12 µM and magenta inclined triangle: 1.5 µM) and varying substrate concentrations while maintaining fixed the concentration of the co-substrates (13 mM for SP, 200 μM for ATP and 100 μM GSH). The enzyme velocities were measured using an end-point assay (see the section Materials and methods for details) and the reciprocal plots are shown for the varying substrate: (A) GSH, (B) ATP, (C) SP and (D) GSP [mono(glutathionyl)spermidine].