Figure 5.
On-target effect of compound 20 in promastigotes and amastigotes of Leishmania infantum. (A) L. infantum promastigotes from the wildtype (WT) or TryS single knockout (sKO) cell line were incubated for 24 h in the absence (white bars) or presence of 7 µM 20 (black bars). The content of free glutathione (GSH) and trypanothione [T(SH)2] is expressed as % relative to samples from non-treated WT parasites. * and **, denote p values < 0.025 and = 0.0052, respectively (two-tailed t-test). (B) Murine macrophages (cell line J774) infected with L. infantum amastigotes from the WT and sKO cell lines were incubated for 24 h in the absence (white bars) or presence of 1 µM 20 (black bars). The results are expressed as relative fold-change in GSH and T(SH)2 content with respect to the corresponding non-treated control from each sample. ***, denotes a p values ≤ 0.001 (two-tailed t-test).