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. 2020 Dec 4;15(12):e0243500. doi: 10.1371/journal.pone.0243500

Fig 5. Live confocal imaging of innate immune responses after microinjection of the different inocula.

Fig 5

Live confocal imaging after microinjection of planktonic bacteria and microbeads (A, B) in the ear pinna of LysM-EGFP transgenic mice at early time points (4–6 hpi). Innate immune cell recruitment towards injection sites was observed between 4.35 (A) to 5.05 hpi (B). Live confocal imaging after microinjection of biofilm-coated microbeads (C, D, E, F) in the ear pinna of LysM-EGFP transgenic mice at early time points (4–6 hpi). Innate immune cell recruitment towards injection sites was observed between 4.15 (C) to 4.45 hpi (D). Images show maximum intensity projections of EGFP (green) and mCherry (magenta) fluorescence. A progressive recruitment of EGFP+ innate immune cells was observed (white empty circles) at the injection sites with cell-bacteria contact areas (filled white arrowheads). *: autofluorescent hair (also in magenta). Filled white arrowheads indicate cell-bacteria contact. Live confocal imaging of two additional experiments at early time points after microinjection of biofilm-coated microbeads, at 4.15 hpi (E) and 4.00 hpi (F). Scale bar: 100 μm.