Fig 7. BbsR9 binds to ftsH, nuoF, and gcvT transcripts via specific GC-rich seed regions.

RNA-RNA EMSAs showing dose-dependency of biotin-labeled BbsR9 binding to wild-type but not mutated, in vitro-transcribed segments of A-C) ftsH, D) nuoF and E) gcvT. Mutated regions correspond to those shown in Fig 6B, and “Dbl” specifies the double ftsH mutant. Red and blue arrows indicate bands corresponding to BbsR9 bound and unbound to target RNAs, respectively. All lanes contained 2 nM biotin-labeled BbsR9 in addition to increasing amounts of the indicated target (2 nM, 4 nM, 8 nM, and 16 nM).