Table 1. Conditions used to prepare B. bacilliformis cultures for RNA-Seq experiments.

Conditions	Medium	Designation	Shift Time	Simulation
pH 7.4, 25°C	HIBB plates	Pl25	2 hours	Sand fly ambient temperature
pH 7.4, 30°C	HIBB plates	Pl30	2 hours	Sand fly ambient temperature
pH 7.4, 37°C	HIBB plates	Pl37	2 hours	Human host
pH 7.4, 37°C with blood gasa	HIBB plates	PlBG	2 hours	Human host
pH 6.0, 30°C	HIBB liquid	pH06	2 hours	Sand fly post-blood meal
pH 7.4, 30°C	HIBB liquid	pH07	2 hours	Human host / sand fly blood meal mid-digestion
pH 8.2, 30°C	HIBB liquid	pH08	2 hours	Sand fly initial blood meal
pH 7.4, 37°C with blood gas	HUVECs in EGM-Plus medium	HUVE	24 hours	Human endothelial cell infection
pH 7.4, 37°C	Human blood	HB37	2 hours	Human erythrocyte infection
pH 7.4, 37°C with blood gas	Human blood	HBBG	2 hours	Human erythrocyte infection

HIBB, Bacto heart infusion blood agar containing 4% defibrinated sheep blood and 2% sheep serum (vol/vol); HUVECs, human umbilical vein endothelial cells; EGM-Plus (Lonza), endothelial cell growth medium containing 2% fetal bovine serum and bovine brain extract.

a Blood gas is comprised of 5% CO₂, 2.5% O₂, and 92.5% N₂ at 100% humidity to simulate human blood.