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. Author manuscript; available in PMC: 2020 Dec 4.
Published in final edited form as: Xenotransplantation. 2018 Oct 12;26(2):e12466. doi: 10.1111/xen.12466

Figure 4. Porcine monocyte phagocytosis of human red blood cells is inhibited by SIRPA-CD47 interaction.

Figure 4.

(A) Schematic of cells and antibodies used. Phagocytosis was only induced between human RBC and porcine monocytes when human RBC were incubated with anti-human CD47 blocking antibody B6H12, showing that human CD47 binds to porcine SIRPA to inhibit phagocytosis.

(B) Human monocyte phagocytosis of human RBCs incubated with B6H12, 2D3, or mouse IgG control antibodies (n=2).

(C) Porcine monocyte phagocytosis of human RBCs (n=2 S pigs, 5 NS pigs) incubated with the same antibodies. Percent phagocytic monocytes are shown for both human and porcine monocytes. Results from four independent experiments shown. Error bars represent standard error. * p < 0.001