Table 1.

List of primers used in this study

Primers	Sequence 5ʹ-3’	Size (bp)	Tm	Tm Multiplex	Multiplex group	Ref.
tetMF tetMR	GCG TAC AAG CAC AGA CTC GT AGC CAT AGC GTA TCC CCT CC	1142	61	64	1	[6]
tetWF tetWR	GAG AGC CTG CTA TAT GCC AGC GGG CGT ATC CAC AAT GTT AAC	168	64			[57]
intF intR	GGC TAC AGA CCG AGT ACC AGC GGA ACT TGA CGT TCG CCA CT	684	61			[6]
ermBF ermBR	GGT AAA GGG CAT TTA ACG AC CGA TAT TCT CGA TTG ACC CA	494	55	60	2	[58]
tetQF tetQR	AGA ATC TGC TGT TTG CCA GTG CGG AGT GTC AAT GAT ATT GCA	167	50			[57]
tet32F tet32R	GAA CCA GAT GCT GCT CTT CAT AGC CAC GCC CAC ATG AT	620	57			[59]
tetLF tetLR	TCG TTA GCG TGC TGT CAT TC GTA TCC CAC CAA TGT AGC CG	267	55			[60]
tetOF tetOR	AAC TTA GGC ATT CTG GCT CAC TCC CAC TGT TCC ATA TCG TCA	515	55	58	3	[61]
tetSF tetSR	GAA AGC TTA CTA TAC AGT AGC AGG AGT ATC TAC AAT ATT TAC	168	50			[57]
tet31F tet31R	CAA TCA CGC CCA AAA GAA TGT GCC ATC CCA GTT TGT	564	53			[62]
tetBF tetBR	AAT AGC CAC TAA ATG GGG CG ATA ACA CCG GTT GCA TTG GT	243	58	56.5	4	[6]
tetKF tetKR	TCG ATA GGA ACA GCA GTA CAG CAG ATC CTA CTC CTT	169	52.8			[60]
tet37F tet37R	ATG GTT CGC TAT TAC TCT AAC ATC AGT CTC ATA TTT CGA CA	170	50			[6]
27 F 1544 R	GAG TTT GAT CCT GGC TCA G AGA AAG GAG GTG ATC CAG CC	approx. 1500	57	-	-	[63]