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. 2020 Dec 2;27(1):1686–1694. doi: 10.1080/10717544.2020.1833381

Figure 3.

Figure 3.

Validation of 3D optical/CT localization of Cy7 labeled mouse albumin (Cy7ALB) directly injected into the brain of a normal mouse. 5 µl of Cy7ALB was injected into right hemisphere. (A) Cy7 epi fluorescent images of 2 different mice 24 h after intracranial injection of Cy7ALB showing accumulation on 2D (left) and 3D OI/CT (right), confirming intracranial placement of infused Cy7ALB in the top mouse but superficial localization in the bottom mouse. Note that the intensity on the epi fluorescent images is heavily influenced by how close to the surface the dye is located rather than amount of Cy7ALB present, due to tissue attenuation of the Cy7 signal. (B) Confirmation of localization of Cy7ALB 3D OI/CT on sections of mouse brain imaged post necropsy. Mice were implanted with Cy7ALB at 2.5 mm depth (top, n = 3) and 1 mm depth (bottom, n = 3) and imaged with 3D OI/CT 24 h after injection (left panels). Immediately after imaging, mice were euthanized and their brains were sectioned and imaged (right panels) to confirm localization of 3D OI/CT images. (C) Coronal 3D OI/CT images of a representative mouse 24 h (top) and 96 h (bottom) after injection of Cy7ALB signal, demonstrating intracranial signal 24 h after injection but not at 96 h, indicating the potential to reimage subsequent infusions. Quantification of the results is shown on the right (n = 4).