Figure 4.

Detection of BBB permeability with systemically injected Cy7ALB. BBB permeability was accomplished using the previously reported intracranial LPS model to induce inflammatory changes. 24 h after stereotactic LPS injection, Cy7ALB was injected IV and imaged 24 h post injection with 3D OI/CT. Coronal images are shown of (A) control with no intracranial injection, (B) control with intracranial injection of saline, and (C) mouse injected intracranially with LPS. Images demonstrate a lack of Cy7ALB in the control brain with an intact BBB, minimal BBB permeability in the saline infused brain and substantial permeability in LPS-infused mouse brain, indicating potential of OI/CT to detect intravenously injected Cy7ALB when the BBB is compromised.