Figure 5.
3D optical/CT visualization of glioblastoma in a mouse brain using systemically injected Cy7ALB. (A) Bioluminescence (biolum) image of mouse with implanted G48a-FLuc cells. (B) Coronal (top) and sagittal (bottom) views of microCT with OmnipaqueTM contrast (left panels) and 3D optical/CT (right panels) 24 h post systemic injection of Cy7ALB. Images demonstrate intracranial Cy7ALB accumulation in the mouse with growing G48a GBM caused by BBB permeability in the region of the tumor, corresponding to the CT contrast accumulation. (C) Tumor to background ratio obtained using Cy7-albumin 3D optical detection (Cy7ALB) and CT with contrast (CT). (D) After imaging, mice were injected with Evan’s blue (EB) dye and euthanized 4 h later to confirm BBB penetration. Photograph of EB and epi fluorescent images of the post mortem brain of a control non tumor bearing mouse (top) along with brain of the GBM-bearing mouse from images A and B (bottom), confirming BBB permeability and Cy7ALB accumulation, respectively.