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. 2020 Dec 3;25(1):104–111. doi: 10.1080/13510002.2020.1857157

Figure 4.

Figure 4.

FLIP knockdown decreased the expressions of FLIP, NLRP3, Caspase-1, IL-1β and IL-18 in LPS + ATP-activated NLRP3 inflammasome. (A) RAW264.7 macrophages were transfected with three different siRNA targeting FLIP, followed by incubation with LPS and ATP. the mRNA and protein expressions of FLIP were detected by real-time PCR and western blot. **p < 0.01vs. si-NC. RAW264.7 macrophages were transfected with si-FLIP-3, followed by incubation with LPS and ATP, the mRNA expressions of FLIP, NLRP3 (B), as well as Caspase-1, IL-1β, IL-18 (C) were detected by real-time PCR. ##p < 0.01 vs. Control, **p < 0.01 vs. LPS + ATP + si-NC.