Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | XL1-Blue | Agilent | 200236 | |
| Strain, strain background (Escherichia coli) | LB2003 |
Parfenova et al., 2006
This paper |
From Dr. I. Hänelt lab |
|
| Strain, strain background (Escherichia coli) | OverExpress C43(DE3) | Lucigen | 60446–1 | |
| Recombinant DNA construct | MthK FL (M. thermoautotrophicum) |
This paper | UniProtKB-O27564 | From Dr. B. S. Rothberg lab |
| Recombinant DNA construct | MthK IR construct | This paper | Delete the DNA region corresponds N-terminal 2–17 amino acid in MthK full length |
|
| Recombinant DNA construct | MthK ∆C construct | This paper | A stop codon was introduced after H117 in MthK IR background |
|
| Peptide, recombinant protein | Thrombin | GE Healthcare | 27084601 | |
| Peptide, recombinant protein | Trypsin type I | Sigma-Aldrich | T8003 | |
| Peptide, recombinant protein | Trypsin inhibitor type II-O |
Sigma-Aldrich | T9253 | |
| Peptide, recombinant protein | Ready-Lyse Lysozyme solutions |
Lucigen | R1804M | |
| Peptide, recombinant protein | OmniCleave Endonuclease | Lucigen | OC7850K | |
| Chemical compound, detergent | n-Decyl-β-D- maltopyranoside (DM) |
Anatrace | D322 | |
| Chemical compound, detergent | n-Dodecyl β-D- maltoside (DDM) |
Anatrace | D310 | |
| Chemical compound, lipids | Soybean Polar Lipid Extract |
Avanti | 541602C |