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. 2020 Nov 16;9:e61265. doi: 10.7554/eLife.61265

Figure 7. B2 NA destabilization leads to increase in expression of B2-SRGs.

Figure 7.

(A) Experimental design for the B2 RNA knock-down cell culture assay employing HT-22 cells. (B) Expression levels of full-length B2 RNA (RT-qPCR) in the B2 RNA KD experiment. Statistical significance (p value threshold 0.05) for anti-B2 less than control (depicted as asterisk, n = 3, unpaired directional t-test, error bars represent standard deviation from the mean). (C) Expression levels (RT-qPCR)_of selected B2-SRGs (see Figure 2—figure supplement 2 and Figure 6D) and the respective negative controls during B2 RNA KD. Statistical significance (p value threshold 0.05) for anti-B2 greater than control (depicted as asterisk, n = 3/group, unpaired directional t-test, error bars represent standard deviation from the mean). Negative controls include five non-B2-SRGs that show no statistically significant difference between the two conditions.