Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Cell line (Mus musculus) |
HT-22 | Millipore Sigma | Cat#SCC129, RRID:CVCL_0321 | |
Sequence-based reagent | Amyloid Beta peptides ( 1-42) | Sigma-Aldrich | Custom synthesis | DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA |
Sequence-based reagent | Amyloid Beta peptides (Reverse 42–1) | Sigma-Aldrich | Custom synthesis | AIVVGGVMLGIIAGKNSGVDEAFFVLKQHHVEYGSDHRFEAD |
Peptide, recombinant protein | Hsf1 protein | Enzo life sciences | ADI-SPP-902-F | Synthesized in insect, human sequence |
Commercial assay, kit | NEBNext Small RNA Library Prep set | NEB | Cat# E7330 | |
Commercial assay, kit | NEBNext Ultra II directional RNA library prep kit | NEB | Cat# E7760 | |
Commercial assay, kit | Superscript III RT | Invitrogen | Cat# 18080093 | |
Commercial assay, kit | Luna universal master mix | NEB | Cat# M3003 | |
Antibody |
Anti-Hsf1
(Rabbit, polyclonal) |
Enzo | Cat# ADI-SPA-901 RRID:AB_10616511 |
WB: 1:1000 |