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. 2020 Nov 19;11:558874. doi: 10.3389/fendo.2020.558874

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Copyright © 2020 Kanwal, Aliya and Xin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of DIP on gut microbiota diversity and abundance in HFD mice. (A) Rank abundance curve, (B) Shannon index, (C) bar graph for Shannon index, (D) observed species, (E) bar graph for observed species showing species diversity, richness, and abundance. (F) Cluster dendrogram and (G) principal coordinate analysis (PCoA) representing the level of similarities or dissimilarities among different treatment groups (n = 4). The data is presented as the mean ± SEM, (n = 4) using one-way ANOVA followed by the Tukey post hoc test. Significant differences were identified at ^####p < 0.0001, HFD group vs control group; ****p < 0.0001, HFD group vs HFD + DIP(L) group or HFD group vs HFD + DIP(H) group. The clustering of PCoA analysis is statistically supported by the one-way ANOVA using ANOSIM test (R = 0.4722, p = 0.002).