Figure 5.

Schematic overview of the co-culture model: SZ95 sebocytes are cultured in a cell culture plate in Sebomed medium for 5 days, HaCaT keratinocytes are cultured in inserts for 5 days in DMEM, after which both are co-cultured for an additional 2 days in Sebomed medium (high calcium concentration), before C. acnes is added to the insert (MOI of 10:1) (A). ΔTEER after 24 and 48 h of infection, compared to uninfected control (dotted line) (B). *p < 0.05, **p < 0.01, ***p < 0.005.