FIGURE 6.
O-GlcNAc elevation affects RVD. (A) HEK 293 Phoenix cells were incubated for 1 h with 100 μM PUGNAc plus 5 mM glucosamine (n = 4) or 0.1% DMSO plus 5 mM glucose as the control (n = 5), initially kept in extracellular isotonic solution and then submitted to hypotonic stress. (B) V/V0 values determined in extracellular isotonic solution and 30 s (swelling) and 160 s (RVD) after exposure to the hypotonic solution. (n) refers to the number of cells. Data are representative of two independent experiments leading to similar results. *p < 0.05, ***p < 0.001, two-way ANOVA with Bonferroni’s post-test. RVD, regulatory volume decrease.