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. 2020 Nov 16;8:599376. doi: 10.3389/fcell.2020.599376

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Copyright © 2020 Ma, Sun, Pi, Wang, Sun, Yu, Shi and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sirt3 over-expression mitigates senescence-associated phenotypic features in natural senescent MSCs and H₂O₂-induced premature senescent MSCs. (A) After Sirt3 over-expression, age-related morphological alterations were evaluated in both senescent MSCs, and the cell areas and the cell aspect ratio were quantified. (B) SA-β-gal staining and quantification were performed. Meanwhile, lentivirus-expressing Sirt3 (LV-Sirt3) was also transduced into H₂O₂-treated MSCs, and its morphological alterations (C) and the ratio of SA-β-gal-positive cells (D) were assessed. Gene expression of senescence-related factor pl6^INK4A (E) and p21^WAF1/CIP1 (F) in MSCs obtained from old rats after Sirt3 repletion. Gene expression of senescence-related factor pl6^INK4A (G) and p21^WAF1/CIP1 (H) in Sirt3 over-expressed MSCs derived from H₂O₂ treatment. Data are expressed as mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. old or H₂O₂.