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. 2020 Nov 20;11:579218. doi: 10.3389/fpls.2020.579218

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Copyright © 2020 Zhang, Yang, Zhao, Yang, Jiang, Walcott, Yang and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of ClWRKY6. (A) ClWRKY6 localization in the nucleus of Nicotiana benthamiana cells. The leaves injected with the Agrobacterium tumefaciens GV3101 strain carrying 35S::ClWRKY6-GFP were sampled and observed using a confocal microscope (20×). DAPI staining was used as a control for nuclear localization. (B) Co-localization observation of ClWRKY6 and AopP. The leaves were injected with A. tumefaciens GV3101 strain carrying 35S::ClWRKY6-mCherry and 35S::AopP-GFP at a ratio of 1:1 and then observed using a confocal microscope (20×). (C) Analysis of ClWRKY6 localization in watermelon. The cytoplasmic and nuclear components in watermelon leaves were extracted, and an anti-ClWRKY6 antibody was used to detect signals. The H3 antibody was used as a positive control for nuclear components.