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. 2020 Nov 25;2020:8396023. doi: 10.1155/2020/8396023

Figure 5.

Figure 5

Silencing PKM2 suppresses MDA-MB-231 cell EMT. (a) Western blot analysis of protein levels of EMT marker proteins in control, Mitapivat, and PKM2-KD groups. GAPDH was used to normalize protein expression (left panel). The protein levels of EMT markers were quantified and compared (right panel). (b) Q-RT PCR analysis of mRNA levels of key EMT-related genes (Snail, Slug, Zeb1, and Twist1) in control and PKM2-KD cells normalized to GAPDH. (c) Relative mRNA levels of vimentin, N-cadherin, and E-cadherin after EMT induction in MDA-MB-231 cells in control and PKM2-KD cells for 48 h. (d) Changes involved in EMT (∗∗∗P < 0.001, ∗∗P < 0.01, NC : P > 0.01).