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. 2020 Nov 26;2020:3648040. doi: 10.1155/2020/3648040

Figure 1.

Figure 1

Comparison of SWFs (D280 and D580 hens) and ASWFs (D580 hens). (a) A–C: H&E staining of the SWFs (D280, D580) and ASWFs; D–F: immunofluorescent staining of caspase3 in SWFs (D280, D580) and ASWFs at physiological state. Red: caspase3-labelled cells. Blue: DAPI staining. Scale bar: 50 μm. (b) A: Western blot analysis for the expression of ER-stress-related proteins (caspase9, GRP78, and CHOP) in SWFs (D280 and D580 hens) and ASWFs (D580 hens). B: the expression of PCNA, CDK2, CCND1, and BAX mRNAs in SWFs (D280, D580) and ASWFs. Values represent the means ± SEM in each group (n = 15). Different lowercase letters indicate significant difference (P < 0.05).