Figure 4.

(a) Effect of H₂O₂ on cell apoptosis in the atretic follicles. A: H&E staining and TUNEL assay in the SWFs (treated with H₂O₂) and ASWFs. Scale bar: 100 μm for H&E staining and 50 μm for TUNEL assay. B-I: Western blot and gray analysis of occludin, BAX, and PCNA expression after H₂O₂ treatment. B-II: the expression of PCNA, CDK2, CCND1, BCL2, BAX, and P53 mRNAs in the SWFs (treated with H₂O₂) and ASWFs. (b) Effect of H₂O₂ on yolk deposition capacity and steroidogenesis in the atretic follicle model by H₂O₂. A: immunofluorescent labels with occludin (green), VLDLR (red), and DAPI (blue) in the histological sections of the SWFs with different concentrations of H₂O₂. Scale bar: 50 μm. B-I: the expression of FSHR, ER-α, CYP17A1, and CYP19A1 mRNAs. B-II: the expression of yolk deposition-related genes VLDLR, PPAR-γ, and occludin. C: Western blot and grey analysis of ER-α and FSHR after H₂O₂ treatment. Values represent the means ± SEM of three replicates in each group. Different lowercase letters indicate significant difference (P < 0.05).