Table 1.
The primers of qRT-PCR.
| Gene name | Accession number | Primer sequence (5′-3′) | Product length (bp) |
|---|---|---|---|
| PCNA | NM_204170.2 | F: GGGCGTCAACCTAAACAGCA R: AGCCAACGTATCCGCATTGT |
97 |
| CCND1 | NM_205381.1 | F: CCTCAAGAAAAGCCGGTTGC R: CTGCGGTCAGAGGAATCGTT |
86 |
| CDK2 | NM_001199857.1 | F: TCCGTATCTTCCGCACGTTG R: GCTTGTTGGGATCGTAGTGC |
183 |
| MTP | NM_001109784.2 | F: GCAGATGGACAGAGTTGGCT R: TTCCCTCTCCTCGCAGTGTA |
93 |
| ApoB | NM_001044633.1 | F: AGGTAGAGGCAGGACGCATA R: AGAATGCTACGTCCCACACG |
268 |
| VLDLR | NM_205229.1 | F: ATGGCCAGGATCGTAGACTT R: TCATTTATCTGAGGAGCAGG |
292 |
| PPAR-γ | NM_001001460.1 | F: CAAGGCAGCGGCAAAATAAC R: GTGCCCATAAATGATGGCCTAA |
187 |
| Occludin | NM_205128.1 | F: CTCTGGGAAGGGCTGAGGT R: GCCTTCCCAAAAAGCCCTGA |
170 |
| BCL2 | NM_205339.2 | F: ATCGTCGCCTTCTTCGAGTT R: ATCCCATCCTCCGTTGTCCT |
150 |
| P53 | NM_205264.1 | F: AACCATTGCTGGAACCCACT R: GCCAGTTGCTGTGATCCTCA |
99 |
| ER-α | NM_205183.2 | F: TAGTTCCGCTCTACGACCTCTT R: AGTTGGTTTCGGTTCTCCTCTT |
106 |
| CYP17A1 | NM_001001901.2 | F: GGAGCTGACAGATGACCACC R: TCTTCTGGACCTCGGGGRAG |
125 |
| CYP19A1 | NM_001001761 | F: CCTCTGCTGGAGATGGTTTT R: GCTGATCCACTTTAGTCACTCTGA |
68 |
| IP3R | KY290442.1 | F: CTACTTGAAGGCGGAAACCCT R: GCCCGCCATGTCTGAAGTAT |
162 |
| MCU | XM_025151715.1 | F: CTGAAATGCATGGCTGCACG R: GTTTGAGGGTGAACTGGCAAC |
164 |
| CAMKII | XM_015288323.2 | F: AGATGAGGATCTGAAAGTGCGT R: TTCCCCAGTGCTTCAGGTTC |
144 |
| MFN1 | NM_024200.4 | F: CGGAGTGAGTGTCCGCTG R: GTGCTTCAGTGGAGATACCGT |
180 |
| MFN2 | XM_015297206.2 | F: GTGGTTGTGTTTTGTTACTTCCG R: AGGGACATTGCGTTTTCAGG |
275 |
| PI3K | NM_001004410.1 | F: ACACGTTCTTGTGCTGGCTA R: TAAGACAAAGGGCACACGCT |
177 |
| GLUT4 | XM_025145523.1 | F: CGTGGGTGAGCTTTCCAGAT R: CATGTAGCGCCCAAAGAACG |
86 |
| IRS | XM_003641084.4 | F: GTCATCCCGCTCTACCAGTG R: TTCTCCGCCAGCATAGCAAA |
110 |
| AKT1 | NM_205055.1 | F: GCGAGAAGGGGAGGAGGA R: CCTGAACTCCAGCAGAAGGG |
272 |
| GSK-3β | XM_004938179.3 | F: ATGGACTAAGGTCTTCCGGC R: CTCTCGCCCGTTTGGTAACT |
166 |
| VTGII | NM_001031276 | F: TTGCAAGCTGATGAACACACAC R: GATTGCTTCATCTGCCAGGTC |
192 |
| OVR | X95100.1 | F: TGAAGCCTGCTGTGACTGTG R: AAGTGACTGACAGGAGTGAGC |
249 |
| GRP78 | NM_205491.1 | F: GAATCGGCTAACACCAGAGGA R: CGCATAGCTCTCCAGCTCATT |
118 |
| GRP75 | NM_001006147.1 | F: GCAGCAGGCTTCCTTGAAAC R: ATTCCGCCCATTTCTGCTCA |
198 |
| FSHR | NM_205079.1 | F: ACCTGCCTGGATGAGCTAAA R: ATCCATGACTTGGCAGGAAG |
136 |
| CALR | XM_025145796.1 | F: GTGGAGACCCCGACAGATTG R: GTGGAGACCCCGACAGATTG |
99 |
| Caspase9 | XM_424580.6 | F: GCTTGTCCATCCCAGTCCAA R: TGCTGCTGACACCTTCACCATTC |
95 |
| β-Actin | NM_205518 | F: ACACCCACACCCCTGTGATGAA R: TGCTGCTGACACCTTCACCATTC |
136 |