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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Brain Res Bull. 2020 Oct 16;165:272–280. doi: 10.1016/j.brainresbull.2020.10.012

Figure 4. Effect of six weeks ethanol consumption on p-nNOS and t-nNOS protein expression in the AcbCo and AcbSh.

Figure 4.

(A) Representative immunoblots for p-nNOS and β-tubulin in AcbCo and AcbSh respectively (upper panel) of the water-control and ethanol groups. Statistical analyses revealed no significant change in p-nNOS protein expression within either the AcbCo or AcbSh of the ethanol group, as compared to the water-control group (lower panel). (B) Representative immunoblots for t-nNOS and β-tubulin in the AcbCo and AcbSh, respectively (upper panel), of the water-control and ethanol groups. Statistical analyses revealed no significant change in t-nNOS protein expression in the AcbCo of the ethanol group, whereas t-nNOS protein expression in the AcbSh was significantly downregulated by chronic ethanol (lower panel). (C) Statistical analyses showed a significant increase in the p-nNOS/t-nNOS ratio following ethanol drinking in the AcbSh, but not AcbCo. The ratio is calculated by dividing the expression of p-nNOS by t-nNOS from the same western blot membranes. Data are shown as mean ± SEM; (*p < 0.05); (n = 8 for each group).