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. 2020 Dec 5;27(3):473.e1–473.e4. doi: 10.1016/j.cmi.2020.11.033

Table 2.

Results of the confirmatory neutralization assay in relation to both screening assays. Samples that tested negative in screening assays were included since they belong to serum pairs (see Methods)

Results of pretesting
Results of confirmation
ELISA CLIA Number of samples NT Number of samples Minimum titre Maximum titre
Negative Negative 23 Negative 22
Positive 1a 1:16 1:16
Equivocal Negative 33 Negative 28
Positive 5 1:16 1:16
Positive Negative 26 Negative 23
Positive 3 1:16 1:16
Negative Positive 5 Negative 3
Positive 2 1:64 1:64
Positive Positive 33 Negative 0
Positive 33 1:64 1:≥ 1024
a

This sample was the first of a pair with an identical neutralization assay titre in the second sample and a conversion from negative to equivocal in ELISA, whereas in chemiluminescent immunoassay (CLIA) both samples tested negative.