Fig. 5.

(a) Viabilities of RBMSC cultured by indirect contact method of coated and uncoated Mg–Sr alloy for 1, 3, 5 days, (b) CCK-8 assay on surfaces of Sr–P and Ca–P coated samples. Staining results and statistical analyses of ALP activities at 7 days with extracts of coated and uncoated Mg–Sr alloy (c, d). Mineralization following treatment of RBMSC with negative control, uncoated and coated alloys for 21 days were identified by Alizarin red S. (e, f). Significantly difference at p < 0.05 is shown with different symbols. (*, # and + represent p < 0.05 when compared with negative control, bare Mg–Sr alloy and Ca–P coating, respectively). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)