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. 2020 Dec 4;10:21228. doi: 10.1038/s41598-020-77962-7

Figure 5.

Figure 5

Maternal administration of rescue-dose AA to WT dams during the lactation period. (a) Experimental protocol of the maternal administration of AA to WT dams during the lactation period. Upon delivery (d2), dams were divided into two groups; one was fed AA-free water, referred to as the N group, and the other was fed 1.5 g/L AA water as the A group. Upon birth, during the lactation period, male offspring were divided into the two groups as above. (b) Plasma AA levels in dams on d16 (n = 6–8 per group). (c) Body weights of N and A offspring from birth to d16 (left panel, statistical analysis using a one-way ANOVA with Tukey’s multiple comparison test) and (d) liver weight on d16 (right panel) (n = 6–8 per group). (e) Plasma and hepatic AA levels in N and A offspring on d16 (n = 6–8 per group). (f) 5hmC levels in the livers of N and A offspring on d16 (n = 3 per group). Statistical analysis using an unpaired Student’s t-test unless otherwise indicated. Data are expressed as the mean ± SEM. *p < 0.05; ** p < 0.01; N.S., not significant vs. A offspring.