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. 2020 Dec 4;10:21273. doi: 10.1038/s41598-020-78087-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Typical optical and fluorescence images of entire co-culture MCF7/MRC5 spheroids at 7 day post-seeding. Concentration of MCF7 cells was (a) 1000; (b) 2500 and (c) 5000 cells per well. MRC5 monoculture fibroblasts formed from 1000; 2500 and 5000 cells per well presented in Panels (a)–(c), respectively. MRC5 fibroblasts were pre-stained with green fluorescent dye (PKH67). Scale bars: 200 µm.