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. 2020 Dec 4;10:21273. doi: 10.1038/s41598-020-78087-7

Figure 3.

Figure 3

Characterization of MCF7/MCR5 co-culture spheroids initiated by (a) simultaneous and (b) sequential seeding of MRC5 cells, taken at day 7. Typical cryosections images, stained with cell viability markers (HES and Ki-67), MCR5 green fluorescence marker (PKH67), and stromal markers (i.e. fibronectin, α-SMA). MRC5 fibroblasts were pre-stained with green fluorescent dye (PKH67). Scale bars: 100 µm.